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Image Search Results
Journal: bioRxiv
Article Title: NAb-seq: an accurate, rapid and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cells
doi: 10.1101/2022.03.25.485728
Figure Lengend Snippet: NAb-seq of two hybridoma cell lines revealed antibody sequences. (a) cDNA library size and amplification. Total RNA from two hybridoma cell lines were extracted and converted to cDNA followed by amplification and barcoding, generating whole transcriptome cDNA libraries A and B. (b) Basecalling in Guppy’s super-high accuracy mode yielded approximately 0.5 million total pass reads. (c) Read length of pass reads varied from ∼400 bp to ∼3000 bp. (d) Sequence analysis of cDNA libraries A and B reveals V(D)J recombination, C gene usage and complementarity determining region 3 (CDR3) amino acid sequence. Consensus calling of antibody transcripts revealed IgG isotype.
Article Snippet: Whole transcriptome cDNA libraries were constructed from extracted mRNA using a
Techniques: cDNA Library Assay, Amplification, Sequencing